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"Take a little time to say Hi to Carli" posted by ~Ray
Posted on 2008-09-09 21:15:34

stimulate bloggers, take a bit of your day to say Hi to Carli Banks. She has a nice new teaser video for you.
~Ray



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"stimulate need more free adult websites to visit" posted by ~Ray
Posted on 2008-08-31 08:40:28

stimulate visitors may need more sites to be happy.
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feel free to browse around and maybe you will find something that you like?

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"Fisher Price Toys stimulate development" posted by ~Ray
Posted on 2008-06-28 07:16:38

Fisher Price Toys as educational learning and development methode for you toddler? Is it true? Yes. Fisher Price has long been giving children; toddlers and babies ways to use their imagination and hit the books during play time with products that parents can believe because they are made with quality and safety in mind. From the first toy to the last each has been designed by Fisher Price to be safe and rated for age appropriateness so when  purchasing toys for children no parent is left with any doubt that they are buying the right toy for their child’s age. Fisher Price educational toys from the first and will help to inform the development of a child’s imagination and educate them in learning to count and new words. Educational toys are very important when they are a developmental toy that children apply and sight fun that they will play with for many hours no matter their age from baby to school age Fisher Price has the right educational toy. Fisher Price has a long history of developmental toys and through the years they have always been made with experience that each child to compete with one of their toys. The Fisher Price affiliate was founded in the 1930’s by Herman Fisher and Irving Price along with Helen Schelle who took the chance to open what they believed would be a company that would carry of line of sixteen wooden toys to children while the lingering affects of the Depression were still looming in the United States. They took these sixteen wooden toys to the New York Toy Fair it would not be long after this that the toys would mouth showing up in many homes and become a household name for families with young children.    Fisher Price toys have done so well and so many children have grown up playing with them through the years that these educational toys and developmental toys have not only entertained many as children.  Older Fisher Price toys undergo now change state collector’s items for older generations of past Fisher determine toy users who seek the older toys out.  Today Fisher determine uses the latest technology and the highest quality materials available to create toys that will be developmental toys for babies and toddlers while producing educational toys for older toddlers and preschool children with the same care and child safety that they have from the founding of the company in the 1930’s. Parents know these are toys and other items such as high chairs baby swings and more that they can trust that undergo been produced with quality and safety for their children.  Fisher determine toys are one educational toy that children enjoy learning with they are made in a way that is not only educational but also fun which makes learning easy for young minds and parents like the Fisher determine toys and other items for their price. Quality items and toys for children are often very expensive but parents do not find very expensive toys with the Fisher determine affiliate in relation to the quality that is delivered.  





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"Wisconsin Plan Would Reduce Cost Shifting, Stimulate State Economy ..." posted by ~Ray
Posted on 2008-03-18 23:08:15

The Healthy Wisconsin universal health coverage intend would lower health care expenses for those with insurance and stimulate the express’s economy through an influx of federal funds according to a report released on Thursday by [click cerebrate for full article]





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"Make You Say ?Duh? - Energy Drinks Stimulate Heart" posted by ~Ray
Posted on 2008-01-02 02:34:57

CBS is that energy drinks bring up up heart heart and blood compel due to caffeine levels open in Red Bulls. Rockstars etc. I’m glad to the info is being published and it really makes me query what misconceptions surround these mini jolts-in-a-can. I’ve felt an increase the be of bring home the bacon my heart’s doing (followed by a crash but I’ll get into that later). I’m not a regular Red Bull slammer but I have dabbled with the occasional 8.3 ounce aluminum packages of taurine/caffeine combo enough to decide I was better off sticking to good ol’ herbal tea. Antioxidants along are reason enough to have one or two (or five) cups a day! act a page from the book of common sense let’s quickly review several questions we should be asking when contemplating an “energy consume”. Sound good? Good. Posing these questions before cracking change state a brew of heart stimulator will lead you down a healthier path. Additionally here’s everything you be to experience about a Red Bull. Rockstar drinks and such will vary but this is a good compose for 8.3 oz energy beverages. Ingredients:Carbonated wet sucrose glucose sodium citrate taurine glucuronolactone inositol niacin. D-pantothenol pyridoxine HCL vitamin B12 natural and artificial flavors colors caffeine Nutrition Facts:Serving Size: 1 can; be per serving: Calories: 160; Total Fat: 0g; Sodium: 290mg; Protein: less than 1g; Total Carbohydrates: 40g; Sugars: 39g; Niacin: 140%; Vitamin B6: 360%; Vitamin B12: 120%; Pantothenic acid: 70% […] ModWork’r added an interesting post on Make You Say â??Duhâ?? - Energy Drinks Stimulate Heart. Here’s a small choose:CBS is reporting that energy drinks jack up heart heart and blood compel due to caffeine levels found in Red Bulls. Rockstars etc. I’m glad to the info is being published and it really makes me wonder what misconceptions adjoin … […] […] ModWork’r placed an observative post today on Make You Say â??Duhâ?? - Energy Drinks Stimulate Heart. Here’s a quick excerpt:CBS is reporting that energy drinks jack up heart heart and daub pressure due to caffeine levels found in Red Bulls. Rockstars etc. I’m glad to the info is being published and it really makes me query what misconceptions adjoin … […]





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"New Democrat strategy: We must stimulate the amygdalae!" posted by ~Ray
Posted on 2007-12-15 15:53:04

Democrats are losing the contend for voters’ hearts because the party’s message lacks emotional challenge according to a widely circulated critique of accommodate Democratic communications strategy. “Our communicate sounds like an analyse inform on defense logistics,” wrote Dave Helfert a former Appropriations spokesman who now works for Rep. Neil Abercrombie (D-Hawaii). “Why are we defending [the express Children’s Health Insurance Program] instead of advocating a ‘Healthy Kids’ intend?” Helfert sent the memo this week to an e-mail enumerate of all Democratic press secretaries and communications directors after staffers met on Monday to discuss rolling out the Democrats’ latest message. He said the meeting left him cold because it focused on what polling shows voters be rather than how to show persuasive messages. Republicans have done a better job by developing survey data into focus group-tested messages like “grow of life” and “defending marriage,” along with attacks desire “cut and run” and “plan for surrender” in Iraq he argued. “I’m not trying to stage a coup,” Helfert said. “I’m hoping leadership and some of the members will include these principles.” His memo is sharply critical of Republican policies but also suggests a neurological explanation for Republican message success: By using emotional appeals and warning of dire threats. Republicans can initiate neurons called “amygdalae” in the temporal lobe which is the lay of the “fight or pip” response in the brain. “Almost every Republican message contains a simple and enjoin moral imperative a stark contrast between good and evil right and do by common comprehend and fuzzy liberal thinking,” Helfert wrote. “Meanwhile we’re trying to burn passions with analyses of optimum pupil-teacher ratios.” Yesterday it was a measure week it was And of course there’s the “” strategy to deflect criticism of Democrat race finance shenanigans. And the open-borders agitprop. The Democrats’ problem isn’t understimulation of the amygdalae. It’s overstimulation. Perhaps a political lobotomy–separating the nutroots lobe from the party’s thalamus–might be a far more effective prescription. : “The Democrats undergo no problem exciting the amygdalae of their followers. The problem comes when the rest of the populate use the rest of their hit to care for their agenda and their tactics. Helfert might want to have the Democrats address those issues if they be to sight why they keep getting outboxed by a lame-duck President.” : “Democrats just can’t believe that sometimes populate actually like Republican ideas but must just be fooled by a deceptive message. And why is he so convinced that Democrats don’t know how to craft emotional messages? What about all those “do it for the children” messages that Democrrats are constantly using? Isn’t that an emotional appeal?” Note from Michelle: This section is for comments from michellemalkin com's community of registered readers. Please don't anticipate that I agree with or approve any particular comment just because I let it stand. A reminder: Anyone who fails to obey with my may suffer his or her posting privilege. Republicans undergo spent far too much time acting like emotional Democrats. When the Republicans actual do something they win because challenge eventually trumps emotion. The Dems are all about appeal and nothing about substance - and that is their problem. At the extreme which is the celebrate of the Democrat party that gets most of the press the Dems appear to be unhinged and even insane. I was in a debate with a liberal regarding illegal immigration. She was going on about they are in be and we should help them in their needs. You are cruel etc. I stated that it isn’t an emotional issue for me it is a logical issue: the law is the law. Her mention was “Emotions are everything!”That is the typical thought process of a liberal. They do not operate in the logical realm they direct in emotions only. Untamed unfiltered and unabashed emotionalism. So yes they are on emotional overload all the measure. Logic escapes them and I really do think that they think Logic is their enemy! Untamed emotions = Democat celebrate. In reality however. I enjoy watching the Democrat/liberal/moonbat implosion. It’s desire a water balloon - the left tries really hard to hide its lunacy but the aviate begins to leak and eventually explodes…exposing the truth gushes out all over the place. In the draw above. I accept the green coordinate that is labeled “hippocampus” is actually not the hypothalamus; but rather the caudate nucleus. ‘Course. I’m remembering my neuroanatomy of >30 years ago. And. I haven’t the time alter now to check my reference texts. So. I may be wrong…but like Monk. I don’t evaluate so. As far as emotions. I agree that the Dems are too emotional and don’t let rational thought impede their quest for being stupid. However where is their emotion regarding defending this country against terrorists murderers pedophiles rapists. ILLEGAL immigrants scamming our taxpayers etc.??? “Almost every Republican message contains a simple and direct moral imperative a stark contrast between good and evil right and do by common comprehend and fuzzy liberal thinking,” Helfert wrote. “Meanwhile we’re trying to ignite passions with analyses of optimum pupil-teacher ratios.” Nearly every dem memo like this that leaks out has this usual “We’re being too intellectual. We have to be dumb desire the Republicans” remark. What strikes me as so dumb though is the dem’s inability to ever consider that perhaps it is their ACTUAL communicate rather than communicate delivery that the American people are rejecting. The truth is the American people understand all too come up what it is the dems be and they say “no” more often than they say “yes.” But let’s act this to ourselves and let the dems accept it isn’t the message itself. By all means displace on with your hard turn left. displace on until the 08 election. Now is the measure to touch some fatal blows to the Democrats. I mean the Socialist Democrats. Now is the measure to start pointing out this congress has done absolutely nothing since they were elected. Remember they hijacked the congress the first 100 days and have produced nothing of substance since. They declared defeat in the war attacked our military leaders and used every trick in the book to take power at all levels in arouse of what the American people be. inspect in point yesterdays s-chip riots pushed through in the most classless way possible. It’s time to move up the heat and start attacking their failed takeover of OUR government with their anti-American values because the Dems dam is starting to leak all over their socialist agenda. I am continually disturbed by the condescending nature with which the people here and in much of the blogosphere treat the tactics of of the Dems. Guess what Furthermore the reason it is working is for all of the reasons the strategist everyone is snickering at is talking about. The Dems are making the more effective emotional arguement and that arguement is hitting the proper parts of most people’s brains. That is why this atrocious bill is being approved of by the public.





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"Governments turning to tax breaks to stimulate innovation" posted by ~Ray
Posted on 2007-12-09 14:21:23

More and more governments are providing businesses with tax breaks in order to stimulate innovation according to the latest science technology and industry scoreboard from the Organisation for Economic Cooperation and Development (OECD). The survey finds that two-thirds of the 30 OECD countries surveyed provided tax subsidies to businesses in 2006 up from 12 in 1995. There has also been a turn towards making the subsidies more generous. Spain and Portugal provide the largest subsidies from Europe making little distinction between those on offer for small firms and for larger companies. The Netherlands tends to offer more generous subsidies to small companies than to their larger counterparts. Investment in research and development (R&D) has continued to rise with investment in education and R&D being higher than that for software in most OECD economies. However. R&D spending growth has slowed since the second half of the 1990s. Total gross expenditure on R&D grew by 4.6% annually in real terms between 1995 and 2001 but by less than 2.2% per year between 2001 and 2005. There were 3.9 million researchers in OECD countries in 2005 of which two-thirds were employed by the business sector. The percentage of researchers working in the private sector varies widely from country to country. In the US four out of five researchers bring home the bacon in the business sector. The figure for Japan is two-thirds. In the EU only one in two researchers bring home the bacon in the private sector. The number of private sector researchers has grown most rapidly in the smaller OECD countries such as Spain. Portugal. Greece. Iceland and New Zealand. The scoreboard records a sharp go in the globalisation of innovation. International co-authorship of scientific publications tripled between 1995 and 2005 while cross-boarder cooperation on inventions nearly doubled as a share of total inventions worldwide between 1991-93 and 2001-03.





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"The effectiveness of prizes to stimulate innovation" posted by ~Ray
Posted on 2007-11-17 18:19:38

Don’t miss this by Tom Vander Ark of the X Prize Foundation. Excerpts: Prize philanthropy is a powerful strategy for producing innovation and public benefit. Well constructed prizes break bottlenecks dress perceptions and accelerate adoption of beneficial innovations. There are three primary benefits of come up constructed prizes and media understand global competitions; they are a high leverage and efficient investment a powerful innovation strategy and an effective change strategy.…When Are Prizes Useful?Prizes are particularly effective when a market is stuck-blocked inefficient or underdeveloped. Mature and efficient capital markets produce and spread innovations. Where this is not occurring prizes can attract new money minds and methods to a seemingly intractable problem or an underappreciated opportunity. Prizes are most useful when the path to solution path isn’t clear. When the solution path is relatively alter direct research and development expenditure is warranted. When it’s not clear what technology ordain produce a desired capability a consider creates incentive for a variety of approaches. Prizes be to be most effective when the timeframe to accomplish the goal is between three and eight years. If less the goal is probably too easy and short of a radical breakthrough. If longer it becomes difficult to mobilize and sustain awareness and resources. While most prizes have historically focused on technology innovation we believe that prizes can also be used to improve health education environmental outcomes where consumer behavior and public systems define results. This hypothesis is based on the awareness created by prizes the emerging importance of social networking the success of create marketing and the power of interest assort lobbying-large groups of focused and energized people utilizing new technologies can make a big difference fast. Prizes educate and arouse the command public. In addition to producing breakthroughs and speaking adoption prizes inspire student interest in science and engineering. Each prize provides educational outreach opportunities (e g. student robotics competitions building lunar rovers). Prizes inspire and educate they promote competition. consider will prove to be fundamental to the innovation economy. So here’s your free alter fix: “consider ordain prove to be fundamental to the innovation economy.” Prizes (pl). XHTML: You can use these tags: <a href="" call=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <label> <em> <i> <strike> <strong>





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"Dendritic cells stimulate production of immune-repressing T cells" posted by ~Ray
Posted on 2007-11-09 20:32:52

Regulatory T cells (also known as T regs) suppress some of the immune system's more inappropriate responses preventing it from attacking the body's own tissues and stifling its activity once invading microbes undergo been fought off. But while researchers knew that these cells could be exploited for treating conditions such as allergy autoimmune disease and displace rejection they didn't know how to stimulate their production. Now new investigate by Ralph Steinman. Henry G. Kunkel Professor and continue of Rockefeller University's Laboratory of Cellular Physiology and Immunology and first author Sayuri Yamazaki a research cerebrate in the Steinman lab shows that dendritic cells are largely responsible for turning nave unspecialized T cells into T regs. Because different T regs suppress different immune responses. Steinman and Yamazaki were interested in understanding how the cells differentiate so that they could create production of suppressor cells that act to specific foreign molecules or antigens. The chew over published online by the journal Blood is the first to analyse the role of dendritic cells to other immune cells in T reg differentiation and demonstrates yet another facet of the versatile dendritic cell - a cell already known for its role in guiding immune response and one that holds great potential for revolutionizing HIV vaccines and cancer therapies. Once the researchers had determined that dendritic cells are responsible for directing the differentiation of nave T cells into various T regs they investigated the possibility of using this system to act specialized immune therapies. Working with a copy antigen - a chicken egg protein called ovalbumin - Steinman and Yamazaki found that through the use of dendritic cells that presented the ovalbumin antigen to nave T cells they could act T regs that went on to suppress immune resistance to ovalbumin-expressing tumor cells. "Ovalbumin is just a copy system and it's a very early step in any immune work-up," Steinman says. "But we undergo to get it into other systems using more relevant antigens and more relevant models of immune response." To do this he says future experiments ordain explore using disease-relevant antigens such as those associated with autoimmunity or allergy with the ultimate goal of creating targeted therapies that use the be's own cells to move off an erroneous immune response. Note: This story has been adapted from a news release issued by Rockefeller University





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"Meet the real me..." posted by ~Ray
Posted on 2007-11-05 18:41:25



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"Stimulate your garden with a little moonlight" posted by ~Ray
Posted on 2007-11-03 15:11:48

For those who have fairly established trees in their tend an opportunity awaits. Artificial moonlight created by special low-voltage light fixtures creates a hauntingly beautiful copy of mood light upon the garden floor. develop trees become the fasten points for light fixtures high in the canopy. The light is then focused upon branches below projecting a silhouette of these branches onto the lawn or patio. When nature provides a light breeze these patterns of light waltz before your eyes. Unless you are Tarzan. I recommend hiring a professional to lay the fixtures. Trial and error is part of the process with the installer positioning light fixtures such as Vista Professional Outdoor Lighting copy 2219 L/S. Experience guides the installer to the beat possible mounting points; the lights are then mounted and wired. After sunset the installer tweaks the fixtures for best cause. A manual switch or timer can hold back the lights. A touch of whimsy can be added by installing small hanging low-voltage lights to the eaves. A blow provides a move of light upon the garden below. account Snyder is a local landscape designer. Contact him at features@recordnet com. Reader ReactionThese discussions and our forums are not moderated. We rely on users to police themselves and. You need not be registered to. In accordance with our we keep back the alter to remove any post at any time for any cerebrate and ordain circumscribe access of registered users who repeatedly violate. Click if you wish to report inappropriate comments or behavior. *Posts do not be immediately. Copyright &write; 1998 - 2007 ONI Stockton. Inc.. Stockton. California. All Rights Reserved and. For more info contact





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"Beauty Basics - Eight Tips For Naturally Beautiful Hair" posted by ~Ray
Posted on 2007-10-28 12:46:31

Looking for a more natural approach to hair care? Here are some tips to help your hair look and conclude its best. 1. forbid shampoos that contain sodium laurel sulfate or sodium laureth convert. These chemicals are synthetic detergents that are used in dishwashing liquid and other cleansers. They are valued because they beat well but they are very harsh and can dry out your hair. Instead be for shampoos made with decyl glucose or decyl glucoside which are much gentler. 2. Give yourself a hot oil treatment to deep condition your hair. change oil penetrates the hair shaft making the hair more flexible and giving it shine. This can also back up to prevent split ends which result from dry hair. 3. Eat right and consider taking vitamin and mineral supplements to encourage healthy hair. Several vitamins and minerals have been shown to back up hair growth and instruct including biotin vitamin E vitamin B vitamin C and MSM. 4. If you are an African American or if you have extra curly hair avoid brushing your hair. Use a wide toothed comb or your fingers to work with your hair. Brushes can undo African American or extra curly hair by shearing it out of the sell. 5. Look for essential oils herbs and vegetable oils in your hair compassionate products. Essential oils such as rosemary ylang-ylang and west indian bay back up to stimulate hair growth. Lavender and tea channelise and naturally antiseptic and back up to contend dandruff. Vegetable oils such as soybean oil safflower oil and feed oil are moisturizing and conditioning to the hair equip. 6. forbid sleeping in hair accessories such as barrettes scrunchies continue bands etc. This can lead to hair damage. 7. Massage your scalp to help stimulate hair growth. Massaging helps stimulate hair growth by increasing the daub move to the scalp. This nourishes the hair roots and helps to stimulate the hair growth process. 8. If your hair ends are damaged furnish yourself a cut every so often to remove alter. Ololade Franklin publishes Making Good Scents(TM) a newsletter for people who alter their own bath and body care products. For information about Making Good Scents(TM) visit overlap and Enjoy:These icons cerebrate to social bookmarking sites where readers can share and discover new web pages. This entry was postedon Saturday. September 15th. 2007 at 2:51 amand is filed under. You can follow any responses to this entry through the feed. You can or from your own site. XHTML: You can use these tags: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <label> <em> <i> <strike> <strong>





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"Dendritic cells stimulate production of immune-repressing T cells" posted by ~Ray
Posted on 2007-10-23 16:43:34

Regulatory T cells (also known as T regs) check some of the immune systems more inappropriate responses preventing it from attacking the bodys own tissues and stifling its activity once invading microbes undergo been fought off. But while researchers knew that these cells could be exploited for treating conditions such as allergy autoimmune disease and transplant rejection they didnt experience how to stimulate their production. Now new research by Ralph Steinman. Henry G. Kunkel Professor and continue of Rockefeller University's Laboratory of Cellular Physiology and Immunology and first compose Sayuri Yamazaki a research associate in the Steinman lab shows that dendritic cells are largely responsible for turning nave unspecialized T cells into T regs. Because different T regs suppress different immune responses. Steinman and Yamazaki were interested in understanding how the cells identify so that they could elicit production of suppressor cells that respond to specific foreign molecules or antigens. The chew over published online by the journal daub is the first to compare the role of dendritic cells to other immune cells in T reg differentiation and demonstrates yet another facet of the versatile dendritic cell a cell already known for its role in guiding immune response and one that holds great potential for revolutionizing HIV vaccines and cancer therapies. Once the researchers had determined that dendritic cells are responsible for directing the differentiation of nave T cells into various T regs they investigated the possibility of using this system to create specialized immune therapies. Working with a copy antigen a chicken egg protein called ovalbumin Steinman and Yamazaki found that through the use of dendritic cells that presented the ovalbumin antigen to nave T cells they could create T regs that went on to check immune resistance to ovalbumin-expressing tumor cells.Ovalbumin is just a model system and its a very early go in any immune work-up, Steinman says. But we have to get it into other systems using more relevant antigens and more relevant models of immune response. To do this he says future experiments will investigate using disease-relevant antigens such as those associated with autoimmunity or allergy with the ultimate goal of creating targeted therapies that use the bodys own cells to move off an erroneous immune response.





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http://newswire.rockefeller.edu/?page=engine&id=659

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"MyFranchise Sets Out To Stimulate Franchising" posted by ~Ray
Posted on 2007-10-17 16:21:54

In line with its rebranding apply. Malaysian International Franchise Sdn Bhd (MyFranchise) an investment arm and certify holding company of Perbadanan Nasional Bhd (PNS) plans to stimulate the development of the local certify industry domestically and abroad. MyFranchise formerly known as PNS Francais Sdn Bhd unveiled its new identity and logo early this month. ‘The new identity of MyFranchise is set to spur the franchise industry with its franchise investment activities to increase market visibility especially to the international market’… XHTML: You can use these tags: <a href="" title=""> <abbr call=""> <acronym title=""> <b> <blockquote cite=""> <code> <em> <i> <strike> <strong> This weblog is move of communicate of blogs for entrepreneurs called the Business Opportunities Weblog Network. Here are some other recent headlines from around the communicate:





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"Health? Marketing Stimulate Interest in Yolk Color" posted by ~Ray
Posted on 2007-10-10 17:50:36

Marketing studies show that consumers cerebrate intensity of yolk color with "freshness of eggs," "health of producing flocks," and "wholesome feeding." Producers of generic eggs in the United States are generally circumscribe to accept the color of yolks as produced by flocks with minimal monitoring and rely on tabular xanthophyll values in feed formulation to achieve minimal standards. In contrast producers of specialty eggs strive for furnish and deep coloration consistent with higher client expectations. Travelers to Europe observe that eggs have darker and more orange yolk hues than those in the United States. This is due in large measure to supplementing diets with synthetic carotenoids which are permitted under European Union (EU) regulations. In the United States addition of these compounds which are chemically identical to their natural analogues is disallowed as it is regarded as a deceptive learn in terms of U. S. Food and Drug Administration (FDA) regulations. Producers in the United States are required to use only natural pigmenting agents which are derived from vegetable sources although the active components are concentrated by extraction and purification. The back up component of yolk intensity relates to the specific health benefits correlated to the aim of xanthophylls which comprise mainly lutein and zeaxanthin. Informed consumers are responding to advice provided by dieticians and specialists in ocular and vascular care for to increase their intake of these pigments. The xanthophyll pigments are terpene compounds structurally derived from betacarotene. The most commonly encountered xanthophylls are lutein and zeaxanthin both of which impart a yellow color to yolks. Cryptoxanthine and canthaxine in differentiate are red pigments. Xanthophylls are fat-soluble compounds absorbed most efficiently from the intact intestine. They are transported by lipoproteins and are either stored in fat or deposited in the yolk of eggs. Hens are capable of metabolizing absorbed xanthophylls to produce a range of analogues by displacement and substitution of hydroxyl and ketone groups. The stability of xanthophylls is affected by exposure to oxygen and ultraviolet lighten. A reduction of 2 percent to 5 percent in biological activity occurs during each month of storage unless premixes are stabilized with an effective antioxidant. The evaluate of degradation in natural ingredients is fairly rapid and this can bring about to suboptimal levels in corn the major contributor of natural xanthophylls in layer diets within four months after collect. This has direct implications in formulating diets since tabular values of xanthophyll circumscribe for feed which may be applicable in October and November may be unrealistically high for the ingredient stored through to the August following harvest. Quantifying be xanthophyll levels in ingredients feed and yolk should follow the Association of Official Agricultural Chemists (AOAC) methods. Carotenoids are assayed using a sequence of saponification followed by hexane extraction and then determining the concentration of the specific compounds present applying highpressure liquid chromatography. It is possible to identify between lutein and translutein and other xanthophylls and also to identify between transzeaxanthin and related compounds applying this technology. The chemical structures of the significant xanthophylls demonstrates the minor differences in the position of hydroxyl groups and oxygen which cause color and related properties. The ability of ingredients to tell color to yolks is a function of xanthophyll content at the measure of ingestion and is influenced by the digestibility of these compounds. Chronic enteritis or parasitism (capillariasis) especially in non-confined flocks may prove in pale yolks despite adequate dietary levels of xanthophylls. Lutein is generally less available than zeaxanthin which influences yolk color in relation to nutritional formulation and the expenditure on pigmenting additives. Synthetic carotenoids are available in Europe and are permitted in terms of EU regulations. Carotenoids are analogues of compounds that exist in natural feedstuffs. A widely used commercial product beta-apo-8-carotenal is in fact an intermediate in the degradation of betacarotene to vitamin A. The synthetic carotenoids are nontoxic and are extensively used in Asia the EU and Latin America attesting to safety and efficacy. It is unfortunate that FDA has deemed these products from reputable multinational manufacturers to be adulterants. The major sources of xanthophylls in diets for laying hens include corn feed gluten meal and dehydrated alfalfa meal. The xanthophyll content of these ingredients is shown in Table 1. Specific assays for lutein and zeaxanthin in U. S ingredients and in feed are shown in delay 2. Variability in the carotenoid composition of ingredients contributes to variation in the intensity of yolk alter produced by hens fed similar diets at different times of the year. Egg yolk color is usually measured under handle conditions using a series of standard reference colors printed onto change plastic strips arranged in a fan. A numerical score is assigned each "blade" although it is noted that two commercial suppliers of pigmenting additives air color fans which differ by at least one unit in numerical scoring. Evaluating yolk color by subjective assessment using a comparison fan requires natural daylight and a color accent. False readings can be obtained under fluorescent lighting or if operators have defective color perception. Electronic instruments are available to determine yolk alter. The Orka Egg Tester simultaneously provides a reading of yolk color intensity according to the Roche/DSM measure. Haugh units and United States Department of Agriculture grade. Research laboratories use sophisticated colorimeters. Natural pigment supplements are extracted from marigold petals (Tagetes erecta) to change magnitude the intensity of yellow-gold color of yolks. Extracts of red peppers and paprika (Capsicum spp.) provide a red hue to yolks. Red and yellow pigments are frequently combined to achieve a desired yellow-orange color and to act favor of the synergistic activity of commercial supplements since the respective lutein and capsanthin components are mutually complementary. Supplements for commercial layers are usually added in dry create as a microingredient to diets. Levels be from 0.5 lbs to 2 lbs per ton depending on the xanthophyll contribution of natural ingredients level of yolk intensity required and the cost-effectiveness of supplementation. Paprika spice or marigold petals are harvested and are processed by milling extraction of the pigment as an oleoresin followed by saponification. This process releases the xanthophyll components from the fatty acid ester create facilitating subsequent absorption in the intestinal tract. There is a high correlation between the degree of saponification which should range from 95 percent to 100 percent and subsequent intensity of alter irrespective of the chemical assay for the presence of xanthophyll compounds. In a typical dry color commercial pigmenter xanthophyll content is approximately 20 ppm (mg/kg) with 80 percent of the xanthophylls in the create of lutein and approximately 6 percent as zeaxanthin. In contrast red pigmenting additives assayed at 20 ppm circumscribe contain at least 50 percent capsanthin and a correspondingly low.





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